Xtreme LC

X-LC: Ultra High Pressure Chromatography

JASCO introduced the world's first commercial ultra high pressure HPLC pump for enhanced separations.

Applications in:

• high-speed and high-resolution separations using small particle sizes typically 1.5 to 2 µm
• Proteomics
• LC-MS

The New JASCO X-LC™ system is completely modular and can be used in any combination.

Column manufacturers have been working to develop packing materials with particle sizes smaller than 2 µm to provide ever increasing resolution for high speed and for separating complex mixtures.

This is currently at the forefront of proteomics applications. The small particle size and narrower columns cause extremely high back pressures resulting in the need for systems to deliver precise flow under difficult conditions. The New X-LC™ system from JASCO can operate at pressures up to 15,000 PSI.

The X-LC™system is entirely modular, typically

• Isocratic or High pressure gradient Binary solvent systems comprising one or two JASCO PU-2080ultra or PU-2085ultra pumps.

• Flow rate ranges are from around 10 µl/min to 10 ml/min including gradient mode.
  Pressures up to 15,000 PSI

• Detection can be either conventional UV using the new JASCO X-LC™ detector with high speed response or increasingly LC-MS for proteomics applications. Both the Micromass MassLynx 4 and Finnigan Xcalibur 1.3 or later can control the JASCO intruments to provide an intregrated solution

The JASCO X-LC™ Series extreme high pressure liquid chromatography is designed to operate at pressures apparoaching 15,000 psi for either gradient or isocratic separations. X-LC™ provides researchers with a powerful tool to use small particle columns while providing efficiency and speed that was previously not possible in a commercial HPLC. All of this while retaining the ability to run traditional HPLC methods.

3085PU/3080PU
3075UV/3070UV
3020FP
3059AS
3067CO

Xtreme Speed

The X-LC™ provides chromatographers with a system capable of reducing analysis times up to 10 × as compared to conventional HPLC.

Xtreme Efficiency

The X-LC™ series provides efficiency far beyond conventional HPLC by utilizing columns packed with small particles less than 2 µm.
This plot shows the relationship between HETP (height equivalent to a theoretical plate) and linear velocity of the mobile phase. A smaller HETP value indicates higher efficiency. The optimum linear velocity, which offers the maximum column efficiency, is shown in the graph. Reducing the particle size offers lower HETP values resulting in higher column efficiency. For columns packed with 1.8 µm particles, when the linear velocity of the mobile phase is increased to two or three times its original value, the HETP value is maintained at a much lower level compared to a similar increase when using columns with larger particles. This demonstrates that the flow rate can be increased and the analysis time shortened without sacrificing the column efficiency. The use of smaller particles, however, requires increased pumping pressure. The X-LC™ is capable of delivering solvents at pressures up to 15,000 psi.