XLC, Ultra High Performance Liquid Chromatography (UHPLC)
XLC general information
Further information
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JASCO’s X-LC provides researchers with a powerful tool to take advantage of newly available small particle size packed columns providing efficiency, resolution, and speed that was previously not possible in a commercial HPLC. All of this while retaining the ability to run traditional HPLC methods. The X-LC series of components is designed to operate at pressures approaching 15,000 psi for either gradient or isocratic separations.
Flexible
Capable of UHPLC and conventional HPLC.
Extreme speed
The X-LC provides chromatographers with a system capable of reducing analysis times up to 10 × as compared to conventional HPLC.
Extreme efficientie
The X-LC provides chromatographers with a system capable of reducing analysis times up to 10× as compared to conventional HPLC.
Modular design
The modular nature of the individual X-LC components allows each chromatography system to be customized to fit the requirements of even the most demanding applications.
- Extremely low system volumes
- Reliable gradient performance
- Wide range of detectors
In the case of the separation of mixtures that contain compounds with a wide range of polarities, isocratic conditions may not provide an acceptable separation. To solve this problem, the composition of the mobile phase is changed during the separation. After sample introduction, the ratio of these solvents is programmed to vary either continuously or in steps, resulting in enhanced separation efficiency. JASCO’s X-LC is easily configured for complete control of both isocratic and gradient methods.
X-LC & MASS-SPEC
The X-LC provides a powerful and flexible platform for UHPLC front-end separations. Configuration options include binary high-pressure gradient and ternary high-pressure gradient formats. Combine this system with standard or high throughput autosampler options, automated column selection valves and a wide variety of optical detectors and X-LC meets the demands of a variety of analytical requirements. X-LC can also be controlled directly by a variety of MS software packages eliminating the need to run multiple software platforms, providing a powerful solution for UHPLC/MS users everywhere.
X-LC Software
Software Control using EZChrom Elite™
The industry standard for client and server chromatography data systems
The X-LC system components are fully controlled by EZ Chrom Elite software. This powerful and flexible data system provides full instrument control for every X-LC configuration. EZ Chrom Elite supports operation in either stand-alone or networked data station configurations. Data collected by EZ Chrom Elite can be analyzed and reported using a wide array of analysis and reporting functions including batch reprocessing and summary reporting. Overlay runs, perform system suitability calculations and output data with a variety of report templates using the intuitive graphical interface. GLP/GMP features in EZ Chrom Elite ensure that X-LC results are securely controlled with a full-featured Audit Trail to track all changes. Multiple user access-level assignment and the Electronic Signature feature provide full compliance with 21 CFR 11 requirements.
X-LC components
X-LC Pumps |
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X-LC pumpsPulse-free solvent pumps delivery operate at pressures approaching 15,000 psiThe X-LC 3085PU solvent delivery unit is designed to operate at pressures approaching 15,000 psi for either gradient or isocratic elution modes. The SSQD (Slow Suction, Quick Delivery) pumping system provides durable, accurate and pulse-free operation. This simple design utilizes only two plungers and two check valves for optimum reliability and ease of maintenance. Versatile programming capabilities allow the use of sophisticated time-based programs for isocratic, binary or ternary gradient elutions. |
X-LC DetectorsJASCO offers an ever-growing range of X-LC and HPLC detectors offering maximum sensitivity and operating stability, which includes the world’s only circular dichroism based detector for chiral detections and the most sensitive scanning fluorescence detector on the market today. |
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X-LC Fluorescence DetectorThe X-LC fluorescence detector is the industry’s most sensitive detector and has an excellent signal-to-noise ratio with proven stability and a wide wavelength range (220-700 nm) for both excitation and emission. Advanced optics, holographic concave diffraction gratings, and non-spherical mirrors are cleverly incorporated in a compact package resulting in extremely efficient and reliable fluorescence detection. |
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UV-Vis DetectorsTo ensure the most sensitive and stable X-LC detection, these models utilize a Czerny-Turner monochromator covering a wide wavelength range from 190 to 900 nm with deuterium and halogen lamps (X-LC 3070UV) and from 190 to 600 nm with a single deuterium lamp (X-LC 3075UV). The excellent optical characteristics and program capabilities of the X-LC 3070/3075UV are combined in one compact package, making the X-LC 3070/3075UV the optimal tool for X-LC UV/Vis detection. To efficiently detect the much narrower peaks that are obtained using X-LC, the X-LC UV detectors are capable of data acquisitions at frequencies up to 100 data points per second. The X-LC 3177UV has a unique design separating it from conventional UV detectors, incorporating a 36-element photo-diode array. The 3077UV offers maximum sensitivity and selectivity to allow simultaneous monitoring of chromatograms at four different wavelengths or three wavelengths and a ratio of two others. |
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X-LC Circular Dichroism Chiral DetectorThe X-LC Circular Dichroism Chiral Detector is an innovative CD (Circular Dichroism) detector for X-LC, using the same technology applied in conventional CD spectropolarimeters. The 3095CD enables highly sensitive and selective detections of chiral compounds. It can simultaneously determine both CD and UV absorption of the sample in the same cell, and determines optical isomer separation and purity. To meet X-LC requirements, the 3095CD features a high-speed sampling rate of 50 data points/sec, for both CD and UV signals, and its specially designed flow cell minimizes peak broadening. |
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X-LC Photo Diode Array Detector (PDA)The X-LC PDA Detector offers maximum sensitivity with ultra high-speed data acquisition and processing. Designed specifically for use in ultra high-speed separations arising from UHPLC applications, the X-LC 3010MD is on the cutting edge of PDA detector development. Display functions such as 3-D chromatograms, contour plotting, peak purity, multi-wavelength chromatograms and spectral search are all supported. |
X-LC Autosamplers |
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X-LC Intelligent AutosamplerThe X-LC autosampler is a fully automatic sample injection system with a minimum injection interval of 30 seconds. It enables greater productivity and the highest possible level of precision. Sampling flexibility with up to 768 well positions (two 384-well microplates) is an option for laboratory automation and combinatorial chemistry. Also available is a micro-vial rack (224 vials) or the standard rack (120 vials) for 2 mL vials. |
X-LC Column Ovens |
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X-LC Column OvenThe X-LC Column Oven is a compact column heater/cooler employing an aluminum block design. Its temperature control range is from -15°C below ambient temperature to 65°C using Peltier elements. |
X-LC Other modules |
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X-LC Dynamic MixerThe X-LC three-solvent dynamic mixer provides efficient mixing of solvents for binary and ternary gradient work at pressures approaching 15,000 psi. |
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X-LC DegasserThe X-LC four-line degasser eliminates dissolved gases in up to four mobile phase inlet lines. |
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X-LC Switching Valves |