LC-4000 series

SFC systems, supercritical Fluid Chromatography

There are many reasons for choosing an SFC system for separations. Supercritical Fluid chromatography (SFC) has often been cited as an ‘alternative’ to Normal Phase HPLC (NPLC), however it is much more than that – SFC is truly an orthogonal technique to Reverse Phase HPLC (RPLC) and offers a much greater capability than simple NPLC separations. Samples that can be separated by RPLC can often be separated by SFC and in many cases with better resolution, higher column loading and faster run times.

Innovative chiral separation technology used in Supercritical Fluid

The effect of the chiral compounds is the key factor in the pharmaceutical industry, and in order to evaluate the enantiomer, the chiral separation is taken up as a main theme. As a solution, SFC is drawing attention from many researchers.

Since the separation capacity of SFC is higher than the one of HPLC, the SFC is a powerful tool for separating the chiral compounds which are hard to separate by using HPLC.

Method development by using SFC screening

Before separating and collecting the target chiral compound, finding the optimum separation condition is required (column, solvent, etc.). In order to create the measurement conditions and measure the samples automatically, JASCO can offer the SFC screening for method development, which has the benefits of laborsaving and operation improvement.

Improvement of sample throughput by stack injection

By shortening the sample injection interval to let chromatograms overlap, the separation in prep mode can be done efficiency. It means that the separation and purification of even a large amount of sample can be carried out with high recovery and high purity in short time.

SFC-4000 – Analytical systems

The physical characteristics exhibited by supercritical fluid include a diffusion coefficient of dissolved molecules that is a hundred times greater than it is in liquid and a viscosity that is at least one digit smaller. A SFC system, which employs such a medium as a mobile phase, can be expected to serve as a separation analysis method that can rapidly perform separation without any degradation in separation efficiency, even at fast flow rates, due to a rapid mass transfer inside the column, when compared with high-speed liquid chromatography that uses liquid as the mobile phase.

SFC-4000 – Preparative systems

The Semi-Prep SFC and Prep SFC systems are applied to separate and purify with high recovery. When carbon dioxide is used as the medium, gasification will occur simply by keeping the separated and fractionated sample at an atmospheric pressure, making this one of the techniques capable of highly efficient refining with few post-processing hassles, such as elimination solvents after preparative isolation. This offers a host of advantages, including cost cuts related to the expense of purchasing solvents and discarding organic solvents among other things.

Unique sample collection mechanism

One of main problems in SFC using CO2 as mobile phase when collecting eluted sample, is that the separated sample is flown apart due to volume expansion (approx. 500 times) of released CO2. In order to improve the collection rate, JASCO has developed a Micro Cyclone Separator (MCS) for Semi-Prep SFC system, and a dedicated fraction collector Prep SFC system.