Supercritical Fluid Extraction
SFE general information
Supercritical fluid extraction (SFE) is a technique that adds a supercritical fluid of an extraction medium to a sample containing a constituent targeted for extraction, using the difference in solubility compared with the extraction medium to carry out the extraction procedure. Using supercritical carbon dioxide as an extraction medium has many advantages and is used in a variety of fields. Extraction by means of supercritical carbon dioxide can be expected to improve efficiency, including shorter extraction times and simplified procedures when compared with extraction techniques that employ organic solvents. In addition, it offers easier solvent elimination and concentration procedures. Since its critical temperature is a low 31ºC or so, it enables extraction at a near-room temperature state or in a carbon dioxide atmosphere devoid of oxygen. This makes it a technique that is ideal for materials that exhibit temperature instability or constituents that are susceptible to oxidation. Furthermore, it has received recent attention as an environmentally friendly extraction technique that does not use hazardous organic solvents, as has been advocated by the green chemistry movement in recent years. Applications for SFE include the extraction of active constituents, including various flavors and medicinal constituents from natural products, docosahexaenoic acid (DHA), advanced unsaturated fatty acids and fatty esters such as eicosapentaenoic acid (EPA), fat-soluble vitamins, and pharmaceuticals. Other applications include the elimination of unwanted constituents, such as decaffeination and desolvation within tablets. It also can be applied to the preprocessing of analysis samples, including HPLC and GC.
Supercritical Fluid Extraction System – Configuration
In this system, a supercritical carbon dioxide pump, a modifier solvent transfer pump, an extraction vessel, a thermostatic bath, and an automatic back pressure regulation valve are connected to the downstream end. The extraction vessel can either be a column or cupped vessel, depending on the state of the extraction sample. There is a wide variety of volume types. Extraction parameters include pressure, temperature, and co-solvent/ volume. These parameters can be changed to set the optimal conditions.
Residual Pesticide Extraction System 1 (50 mL)
Attention is being focused on extraction techniques using supercritical carbon dioxide for trace pesticides left behind on food products and in soil. Residual pesticide extraction systems that use supercritical carbon dioxide are a topic of interest at present. JASCO has a lineup of residual pesticide extraction systems equipped with these characteristics.
- Simple operation
- Quick extraction - about 30 to 60 minutes per sample, which is one-third to one-forth the processing time of conventional techniques
- Eliminates trace pesticides - the extract is collected in a trap column
- Minimal extract cleanup - also roughly separates impurities in trap column
- Blockages and contamination are minimized - the trap column and piping is washed each time
- Low running costs - carbon dioxide is highly pure and inexpensive
- The system is safe and environmentally friendly because carbon dioxide is non-toxic and nonflammable.
- Capacity of extraction vessels: 10 mL or 50 mL. The 10 mL vessel is ideal if operability is the focus. The 50 mL vessel is a better selection if the focus is on sample processing volume or analysis sensitivity.
- The 50 mL vessel offers simple one-touch attachment and removal.
- One to six vessels can be processed at a time. JASCO offers the single vessel type or the six-vessel type depending on the frequency of samples being measured.