Applications - Jasco Benelux

Micro-LC system: applications

Chromatogram of Cytochrome C Tryptic Digest

Trypic digest is commonoly used to evaluate performance of micro HPLC system. Peaks of the digest were detected with excellent signal-to-noise ratio at 215 nm wavelength due to optimized UV flowcell designed. High-precion solvent delivery system enabled stable gradient profile offering a series of sharp fishbone like peaks.

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Cytochrome C tryptic(150 pmol) digest was separated on a micro column C18(0.3mmI.D.x 150mm).

Flowrate : 5µL/minMobile phase : A=0.05% TFA in H2O; B=0.05% TFA in CH3CN
Gradient conditions : A/B95/5-50/50(80min, linear gradient)
Column temperature : 40ºC
Detection : UV = 215nm

Analysis of Bisphenol A using Online Pre-Column Concentration Method

Bisphenol A is known as one of endocrine disrupters exists in environment and to give unwanted biological effects even at low concentration below ppb. The standard water prepared contains 1 ppb each of Bisphenol A and related compounds. 200 µL of the standard was injected to the system. All peaks were detected with excellent signal-to-noise, confirming that the system meets the requirements of environmental water analysis. The system can also be used in biological analysis of proteins and peptides.

Flow rate: 5.0 µL/min
Columns:
Pre-column C1; C18 column (0.32 mm I.D. x 35 mm)
Analytical Column C2: C18 column (0.32 mm I.D. x 150 mm)
Mobile phase:
A= H2O/CH3OH (45/55)
B=CH3OH
Pre-concentration solvent: C=H2O
Flow rate: 25.0 µL/min
Column Temperature: 40ºC (Analytical Column)
Detection: Ex.=275 nm, Em.=305 nm
Gain: x 1000, Response: 5sec (Digital), 75 µm Capillary flow cell

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Columns for Capillary LC; Wide range of applications

Separation of a trypic digest from Cytochrome C by gradient elution method at 5µl/min flow rate

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