integrated HPLC, UHPLC and preparative systems
The LC-4000 Series is the latest in a long history of innovative HPLC systems developed by JASCO reaching all the way back to the start of commercial HPLC in the early 1970s. The concept of the integrated LC-4000 series HPLC provides key separation platforms at 50MPa, 70MPa and 130MPa which correspond to conventional HPLC, the increasingly popular Rapid Analysis (RHPLC) and sub 2um UHPLC, respectively.
Advances in Solvent Delivery
For over two decades JASCO analytical HPLC pumps have employed an asymmetric twin-piston solvent delivery system SSQD (Slow Suction, Quick Delivery) which provides significantly better flow and pressure profiles than the commonly used conventional twin-piston reciprocating design. In the LC-4000 series, SSQD has been re-developed, with a completely new solvent delivery mechanism ExReFT (Extremely Reliable Flow Technology) offering the highest stability in solvent delivery across the entire analytical flow rate range used in the PU-4180/85 RHPLC and PU-4280/85 UHPLC pump models.
Pioneering Optical Design
As a pioneer in optical spectroscopy, JASCO has access to optical designs that few can match. Adopting designs from some of our more powerful spectrometers we have developed a range of HPLC optical detectors with unrivalled performance, like the new class leading FP-4020 fluorescence detector with S/N of over 2300:1, the latest high-speed refractive index detector with micro cell optimized for RHPLC and UHPLC and a completely revised circular dichroismdetector for chiral chromatography.
New Technologies for Method Development
With the range of column technologies becoming ever more diverse – the traditional 5um fully porous particle is finally losing ground to some of the newer technologies such as the new superficially porous solid core (Coreshell) and the wide variety of sub-2um particles, the requirements for method development are becoming more demanding. The LC-4000 series has a number of newly developed accessories to help you make better separations faster, with accessories such as, flexible and stackable column ovens in a range of sizes to accommodate analytical to preparative columns and multiple columns at every scale and with options for integrated 6 or 10 solvent and column selection valves.
Compact and Easy to Use
Despite the extra power delivered by the LC-4000 series HPLC, the standard footprint is only 300 mm wide, a standard system requires only a very small amount of bench space. For easy user-maintenance, all LC-4000 instruments feature front-access for replacing consumables such as pump components and light sources, even the autosampler consumables such as the syringe parts are easy to access. For those users who require front-panel control, the LC-4000 series retains the popular keypad and display (with a backlit LED display as standard). Keypad control can still be configured for convenient override when under PC control.
The HPLC systems are coupled with ChromNAV 2.0 data system to offer both HPLC and spectral data handling for most of the detectors even with the dual wavelength UV detector.
ChromNAV 2.0 CFR is available for 21 CFR Part 11 compliance. User privileges are set by four different security levels. Three types of electronic signatures (Created, Reviewed, and Approved) are available. Audit trail function records and archives all operations and file modifications.
HPLC System Configurations
The LC-4000 series HPLC analytical and preparative HPLC systems have a number of common features:
- 300 mm wide footprint
- Each system component is completely stackable into a modest height
- The system is fully integrated with complete system monitoring
- All user replaceable components are accessible through the front panel
- Vertical leak/drain system to channel away solvent
- Integrated earthquake securing option
- All detectors include 100 Hz acquisition with interchangeable flowcells
- Conventional HPLC and RHPLC Analytical Systems up to 70MP
Conventional HPLC and RHPLC Analytical Systems
The LC-4000 Series 70 MPa system has been designed to future-proof your HPLC requirements. The LC-4000 70 MPa system can be used with conventional 3 and 5 μm particle size analytical columns at typical lower pressures around 10 to 20 MPa (1500 to 3000 psi) and can also be used with smaller particles such as shorter length UHPLC columns and superficially porous (SPP) or Coreshell that require slightly higher pressure solvent delivery to provide optimum linear velocity through the column.
UHPLC System Configurations
Ten years after the new segment of the HPLC market, UHPLC has found a niche for those that need to run a large number of samplers or get results quickly. Pioneering column technology is pushing the particle size even smaller. The near 20,000 psi operation of the LC-4000 130 MPa is designed to take advantage of the separation efficiency of the very small particles couple with longer and narrower columns. The LC-4000 130 MPa system incorporates many of the same features as the 70 MPa system, but with materials designed to withstand higher pressures.
Preparative HPLC Systems
The LC-4000 preparative HPLC systems includes three platform options:
- Up to 20 mL/min for columns up to 21.2 mm ID
- Up to 50 mL/min for columns up to 3 cm ID
- Up to 120 mL/min for columns up to 5 cm ID
Large column oven for multiple preparative columns and optional automatic column switching
Open-bed fraction collector with sample trays for tubes, plates or large containers
ChromNAV-FC for fraction control using time, threshold or slope from up to 4 different signals.