Fluorescence: The FP-8000 series
Fluorescensce general information
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Designed with the latest technology, the JASCO FP-8000 Series spectrofluorometers incorporate the highest sensitivity, fastest spectral scanning capability and excellent analysis-oriented functionality offering integrated solutions for advanced materials research and biochemical analysis applications. To meet the most stringent analysis demands, a variety of accessories are
available for integration with a range of sophisticated control and analysis applications available in the user-friendly Spectra Manager™ II software to offer a flexible platform for any fluorescence and phosphorescence application.
- Highest sensitivity up to 5000:1, RMS
- Fastest scan speed of 60,000 nm/min
- Wide dynamic range grater than 6.5 orders of magnitude
- Automatic higher-order diffraction cut filter
- Automatic accessory identification with non-contact type RFID technology
- Rapid 3D spectra measurements
- Expanded features for phosphorescence measurements with lifetime measurements > 1 millisecond
The wide dynamic range is particularly suitable for luminescence measurements. It is obtained using the Auto-Gain and Auto-SCS features, automatically adjusting the detector sensitivity for optimum measurements.
AUTO GAIN let the user Collecting data with an optimized S/N throughout the entire scan range for optimized spectra or time course measurements. It is obtained by automatic gain adjustment due to fluorescence intensity change.
AUTO SCS is effective for fixed wavelength measurements and quantitative analysis, Auto-SCS makes it possible to create a calibration curve for a wide range of concentrations without modifying the instrument measurement parameters.
Four different FP-8000 Series models offer the optimum solution for fluorescence and phosphorescence applications.
FP-8200 : General use model for routine fluorescence analysis
FP-8300 : Versatile model with additional features for Bio applications
FP-8500 : Research model offering the ultimate performance
FP-8600 : NIR model for materials analysis research
A variety of accessories are available for temperature control and specific application requirements.
A high-speed chopper for the FP-8300/8500/8600 offers phosphorescence spectrum measurements as well as advanced phosphorescent lifetime and quantitative analysis measurements. The PMU-830 liquid nitrogen sampling accessory can be added for additional flexibility.
The ATS-826 (FP-8200) or ATS-827 (FP-8300/8500/8600) automatic titration accessory offers two independently controlled syringes. This accessory is a powerful tool for monitoring protein denaturation and ligand binding experiments under temperature control with a thermostatted cell holder. The FDP-837 automatic polarizer allows fluorescence depolarization measurements by using linearly polarized light. With a thermostatted cell holder and the auto-depolarization program, fluorescence polarization can be monitored while changing the sample temperature.
The CTH-807 water-thermostatted cell block option (for all models of the FP-8000 Series) can be easily and quickly installed by replacing the front cover of the standard sample holder. A range of optional water- or Peltier-thermostatted cell holders for single or multiple samples are available for accurate temperature control.
Stopped flow accessory
The SFS-850 Series Stopped Flow accessory for the FP-8300/8500/8600 instruments employs a high-efficiency mixer featuring exceptionally fast mixing compared to conventional mixing systems. The innovative cell design places the cell directly into the sample compartment eliminating light-flux attenuation by optical fibers and resulting in excellent signal to noise ratio and long term performance. An optional Peltier temperature control module is available for both the syringe drive and cell units.
One drop fluorescence measurement
The SAF-850 (FP-8200) or SAF-851 (FP-8300/8500/8600) One-Drop measurement accessory is a dedicated module for rapid measurements of micro-volume samples. Simply place a droplet of sample on the disk cell to obtain a measurement of the sample.
The FMP-825 microplate reader for the FP-8300/8500/8600 instruments allows automated high-throughput and high-speed analysis using a micro-well plate. Temperature control is optionally available.
Automatic titration accessory
The ATS-826 (FP-8200) or ATS-827 (FP-8300/8500/8600) automatic titration accessory offers two independently controlled syringes. This accessory is a powerful tool for monitoring protein denaturation and ligand binding experiments under temperature control with a thermostatted cell holder.